Manual restraint and common compound administration routes in. This pdf is available for free download from a site hosted by. Intravitreal injection of normal saline induces retinal. Systemic injection of neural stemprogenitor cells in mice. Then, purified dna fragment is microinjected into 200 fertilized eggs of c57bl6 mouse strain for producing founder mice f0. Prior to surgery, the mice are housed for 7 days for acclimation and observation.
The radionuclide, 212 pb, has been a focus of investigation for radioimmunotherapy rit 1,2,3,4,5,6,7,8,9,10,11,12, as well as a supply of cytotoxic. Test substances, solutions and equipment should be prepared aseptically and free from pyrogens, especially for parenteral injections. How many of the mice developed inflammation but not nausea. Intraperitoneal injection an overview sciencedirect topics. Retroorbital injections in neonates have also been mentioned 8,9 but, to our knowledge, not described in detail. At 1, 2, 4, or 8 hours after nac injection, the mice were anesthetized and blood was drawn into 1. Sep 23, 2015 restraint in animals is known to cause stress but is used during almost all scientific procedures in rodents, representing a major welfare and scientific issue. Intrahepatic injection of adenovirus reduces inflammation and. Treatment dose vehicle injection source body weight scheme. Interspecies pharmacokinetic modeling of subcutaneous.
Recommended perioperative analgesic protocols for mice and rats mild pain preemptive1 once lidocainebupivacaine as local infiltration postsurgical drug buprenorphine, morphine or oxymorphone frequency once mild to moderate pain option 1 preemptive once lidocainebupivacaine as local infiltration and buprenorphine, morphine or oxymorphone. Cage changing basicsstatic microisolator cages info. We have used this method to inject substances into hundreds of adult mice, without incident. Get like 1020 cd1 mice, be sure to warm up mice with a 250 w infrared lamp at about 1 ft distance until their tail vein are real dilated. One level involves the housing of mice in a cage as described above. At 24 hr after injection, ipsilateral hemispheres show stable hematoma volumes figure 4.
Links to pubmed are also available for selected references. Generation of mutant mice by pronuclear injection of. Evaluation of mouse tailvein injections both qualitatively. This policy addresses issues related to administration of compounds within a protocol with the goal of facilitating the procedure for the person administering the compound as well as minimizing discomfort and supporting wellbeing of the animal. When a relatively low dose of virus was used, iv administration. May 23, 2014 to investigate the effect of dose level and anatomical site of injection on the pharmacokinetics of rituximab in mice, and to evaluate the utility of a pharmacokinetic model for describing interspecies differences in subcutaneous absorption between mice and rats. Considering the risks and safety of intraperitoneal injections.
Pdf urinary corticosterone levels in mice in response to. At all times, the mice are allowed free access to food and water. Two levels of barrier housing of mice are available. It seemed to take george some time to free his words. Quantitative assessment after injection, the syringes and tubing line with residual radiotracer were measured in the dose calibrator to determine the net injected amount of radiotracer.
Im injections are not recommended and should be avoided whenever possible due to the lack of muscle mass in the mouse. Those trained persons listed on an approved animal care committee protocol performing the procedure. Cell proliferation assays provide a critical piece of the puzzle when evaluating cell health, genotoxicity, and the efficacy of anticancer drugs. The water provided to the mice is treated to make it as pure as possible. Pdf virulence factors involved in the intraperitoneal. Histological investigations revealed multiple vacuoles in photoreceptor outer segments and rpe cells. The lesions recovered over time and by 3 weeks post injection the majority of lesions vanished in eyes receiving 1. Control mice were treated with vehicle by treated groups was 1, 11. Intraperitoneal ip injection in rats and mice sop ubc animal. Make sure one of the animals hind legs is free and stabilized for the injection.
Evaluation of a novel technique for intraperitoneal injections in mice. Muscle sarcomas and alopecia in aj mice chronically treated with nicotine valentin galitovskiy a, alexander i. Pharmacological inhibition of type i interferon signaling. Hitting the sciatic nerve can cause pain, lameness, and possible paralysis. Mouse injections biomethodology for laboratory mice. Intravitreal and subconjunctival melphalan for retinoblastoma. On the next day of final injection day 10, mice were sacrificed at zt 0, 6, 12, and 18 n35 for each time point. The animals used are female c57bl6 mice taconic inc. Intraperitoneal injection in the mouse although widely used as a means of administering substances, particularly injectable anaesthetics, this is an inherently unreliable technique, since inadvertent injection of some material into the gut, abdominal fat and subcutaneous tissues is a relatively frequent occurrence. Pull back on the plunger to ensure negative pressure prior to injecting. Acute intravenous injection toxicity study of escherichia. Volume and frequency of administration the injection volume is limited by any toxicity of the substance and by the size of the mouse.
The laboratory mouse handbook of experimental animals usp. The animals are anesthetized with avertin 1 g 2,2,2tribromoethanol dissolved in 0. Signal at the injection site will be detectable at day 0. Urinary corticosterone levels in mice in response to intraperitoneal injections with saline. The most accurate cell proliferation assays directly quantitate newly synthesized dna by following the incorporation of a deoxyribonucleoside analog that contains a detectable tag. Pdf download for impact assessment of tailvein injection in mice. Intrastriatal injection of autologous blood or clostridial.
Pro and antiinflammatory cytokines release in mice injected. Administration of drugs and experimental compounds in mice. To protect your account, the ucla single signon service prevents old or previously used login pages from use. Female mice were injected with 10 iu pregnant mares serum gonadotropin pmsg by nintraperitoneal injection, and then with 10 iu human chorionic gonadotropin hcg after 2 days of pmsg priming. Muscle sarcomas and alopecia in aj mice chronically. Prestudy body weights of the mice were recorded before injection with the 212 pb solution, then at 7 and 90 days just prior to euthanasia table 2.
The authors have developed a new method for administering intraperitoneal injections in mice, with the. Well, you aint petting no mice while you walk with me. Lhbetatag transgenic mice were treated with a single 1 mcg intravitreal injection of melphalan, 100 mcg subconjunctival injection, or semiweekly 10 mcg subconjunctival injections for 3 weeks. Jci nacetylcysteine reduces the size and activity of. Injected substances and the needlessyringes used to inject substances must be sterile.
Full text full text is available as a scanned copy of the original print version. There are several routes of administration available in the mouse and rat. All animal users performing ip injections in rodents must. Intravenous iv administration in mice is predominantly performed via the. At the end of experiment, mice are euthanized and selected tissues are analyzed by ex vivo. When 100 mice were injected with a certain chemical, some developed inflammation at the site of the injection and some developed the condition of nausea. Manual restraint and common compound administration routes in mice and rats. Next, we examined the acute effect of insulin on the impairment. Jci acetaminopheninduced hepatotoxicity in mice is.
Saline or nac solution was injected through the tail vein. After injection, the mice were anesthetized with approximately 1. Generation of mutant mice by pronuclear injection of circular. Balbc female mice injected intraperitoneally presented sensibility to cdt, with changes in specific signs, blood biochemical and inflammatory mediators. Intraperitoneal injection in the mouse procedures with care. Then, purified dna fragment is microinjected into 200 fertilized eggs of c57bl6 mouse strain for. Refinement of intrathymic injection in mice request pdf. Therefore, injection of the drug or drugcontaining devices directly into the vitreous cavity allows high levels of drug to be achieved in the posterior segment of the eye retina and choroid, improving the efficacy of therapy for vitreoretinal diseases. Hold the foot firmly, swab the site with 70% ethanol, aspirate and inject the material with the needle bevel up. Potential complications for all routes of injection include infection, local irritation, pain and damage to surrounding tissue. We prefer the injection route described here because it is clinically relevant, able to be performed by a single experimenter with no special equipment requirements and allows for a wide range of. The effects of crotalus durissus terrificus venom cdt were analyzed with respect to the susceptibility and the inflammatory mediators in an experimental model of severe envenomation. The most used method is intraperitoneal injection, because the amount of administered compound can be better controlled, but delivery by oral gavage is also possible.
Intraperitoneal injection in mice research at penn state. Increased erythropoiesis in mice injected with submicrogram. Restraint in animals is known to cause stress but is used during almost all scientific procedures in rodents, representing a major welfare and scientific issue. Method to perform iv injections on mice using the facial vein. We purify dna fragment for microinjection from plasmid dna provided by the customer. Mice are exposed to a lightdark cycle approximating 1212 h. Intramuscular injection in the rat procedures with care. Impact assessment of tailvein injection in mice using a modified. Balbc mice were injected with adluc ih or iv at a dose of 109 c pfu or 1010 d pfumouse. Gmat injecting mice when 100 mice were injected with a. Mar 27, 2014 intravitreal injection of ns dosedependently induced localized retinal lesion in mice.
Intraperitoneal ip injection in rats and mice sop page 4 of 6 mouse rat 6. The intravenous injection of neonatal rats and mice is an exacting procedure. Clickit plus edu proliferation kits for imaging and flow. When you do injection, put mouse inside a mouse restrainer, pull the tail so the mouse will not move, use a 27 g needle and real small angle near parallel for the entry to prevent a punch through for the. This is consistent with the trend of greater effect on the nervous system of.
Alternative injection routes for neonate mice have been published by us and others including retroorbital, intrajugular and intraperitoneal 10,15,22. Use your thumb and forefinger to make a tent of skin over the scruff. Nov 27, 20 the pronuclear injection of circular plasmid expressing hcas9sgrna complex is a rapid, simple and reproducible method for targeted mutagenesis. Lab techniques in mice free download as powerpoint presentation.
The injection of 3g recombinant murine epo could not be detected beyond 6 hours postinjection, suggesting a rapid halflife, in agreement with studies that found a halflife for exogenously delivered epo in mice of about 2 hours. The pronuclear injection of circular plasmid expressing hcas9sgrna complex is a rapid, simple and reproducible method for targeted mutagenesis. Crotalus durissus terrificus venom cdt were analyzed with respect to the susceptibility and the inflammatory mediators in an experimental model of severe envenomation. For mice housed under sterile conditions, the water is autoclaved. Daily injection of insulin attenuated impairment of liver. Tamoxifen administration to mice jonathan whitfield,1,2 trevor littlewood,3 and laura soucek1,2,4 1. Administration of compounds plays a large part in experimental design using animals. Good practice administration routes and volumes for mice, rats, and rabbits are indicated in table 1. Administration of recommended volumes considered good practice and possible.
A maternal transcription factor, junction mediating and. Major article pharmacological inhibition of type i interferon signaling protects mice against lethal sepsis lien dejager, 1,2so. T h e s ta n d a r d iz ed l i g ht co n d i t i on s i n t h e a ni m a l fa ci l i t i e. This article is from asian spine journal, volume 8. Rituximab serum concentrations were measured following intravenous and subcutaneous administration at the back and abdomen of mice. The sciatic nerve runs along the caudal back length of the femur and must be avoided. Six to 8weekold wildtype c57bl6 mice and littermate controls were used in these experiments.
Pdf evaluation of a novel technique for intraperitoneal. If there is negative pressure, proceed with the injection. Intravitreal injection is an accepted route of drug delivery for the management of retinal and choroidal diseases. Intramuscular injection in the rat in the rat and other small rodents, the very small muscle mass makes intramuscular administration both technically difficult and painful for the animal because of the distension of the muscle. Recommended needle sizes and injection sites for various species are indicated in table 2. The injection of substances directly into the body requires strict asepsis to avoid complications. We think that retroorbital injection is an easy and reliable method for intravascular delivery of many agents. Mortality should be expected to occur between 10 25% of collagenaseinjected. Insert the needle, bevel up, at the base of the tent.
Get a printable copy pdf file of the complete article 285k, or click on a page image below to browse page by page. Clickit plus edu proliferation kits for imaging and flow cytometry see a list of the products featured in this article. Five days after stz injection mice were received saline or insulin 5 iukg at zt 11. There is substantial reduction in apapinduced liver injury and hemorrhage in mice receiving aspirin. Intraperitoneal injection in the mouse although widely used as a means of administering substances, particularly injectable anaesthetics, this is an inherently unreliable technique, since inadvertent injection of some material into the gut, abdominal fat and subcutaneous tissues is a. Being able to safely and effectively restrain mice and rats is an important part of conducting research. Download fulltext pdf virulence factors involved in the intraperitoneal injection of adult mice with vibrio cholerae article pdf available in infection and immunity 2. Method to perform iv injections on mice using the facial. Mice were on aspirin or regular drinking water for 3 days prior to apap injection. An uniform, reflection free illuminati on is essential for succe ssful i. The injection volume should be carefully considered according to the available route. Administration by injection research at penn state. Recommended needle sizes and injection sites for various species are indicated. Mice are placed onto black paper in the ivis imaging box and imaged dorsally.
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